NMR Methods for Labeled Proteins

Topics:

Overview of NMR experiments for protein structure determination.
Introduction to triple-resonance experiments.
Bruker software for biomolecular applications.
Set-up and acquisition of triple-resonance experiments.
Introduction to shaped pulses.
Recent methodological developments in bio-NMR

Remarks:

This is a course for experienced users of Avance spectrometers working with labelled protein samples. The background for choosing and setting-up 3D-triple-resonance experiments for proteins is given. During the practical sessions representative spectra such as 3D-¹⁵N-NOESY-HSQC, HNHA, HNCA, HNCOCA, CBCA(CO)NH and HCCH-TOCSY are acquired. The implementation of the shaped pulses needed for biomolecular NMR and the use of adiabatic pulses, is covered.

Prerequisite Qualification:

Participation in the course Advanced NMR Methods or corresponding knowledge.

Date and Place:

September 29 - October 1, 2010 in Fällanden/Zürich Registration form

Course Cost and Duration:

Course Cost¹	Duration	Language
€900.00 (incl. documentation, coffee breaks and lunch)	3 days	English

Course Coordination:

Bruker BioSpin AG
Mrs. Svetlana Startseva
Industriestrasse 26
CH-8117 Faellanden
Phone: +41-44-825 95 42
Fax: +41-44-825 95 50
E-Mail: svetlana.startseva(at)bruker-biospin.ch

¹Prices are subject to change without notice