Applications - QCI CryoProbe

Versatile Quadruple Resonance CryoProbe for Structural Biology Applications

QCI CryoProbe

New, versatile proton-optimized quadruple resonance NMR ‘inverse’ probe, featuring four fully independent channels (plus lock channel) for simultaneous decoupling on multiple nuclei such as 13C, 31P and 15N - all without changing probe.

Applications

  • RNA/DNA studies
  • Proteomics
  • Metabolomics
  • Small molecules
Protonless HiRes Bio-NMR

Protonless HiRes Bio-NMR

Protonless HiRes Bio-NMR: 3D CBCACO-IPAP of 1mM Ubiquitin 15N/13C labeled, taken at 600 MHz (ns=2,TD=1024x64x256, experiment time 10 hours)

2D 13C observe TOCSY

2D 13C observe TOCSY

2D 13C observe TOCSY with 31P & 15N & 1H decoupling, 4 mg 15N/13C labeled RNA 14-mer, experiment time 40 minutes

1H-15N NN-COSY

1H-15N NN-COSY

1H-15N NN-COSY (H Bond correlation) of 4 mg (~2mM) 15N/13C labeled RNA 14-mer, taken at 600 MHz (ns=8, td= 2kx128, experiment time 25 min, te= 283K).

*RNA standards by Silantes GmbH

Unbuffered male urine

Unbuffered Male Urine Sample

Sample: 350 µl unbuffered male urine in shaped sample tube

 

Top: Presat ns = 8

Left: HSQC aromatic region

Right: 2D J-RES aliphatic region