Biological Tissue Analysis

Z-Gradient TXI hr-MAS Probes and Accessories

High Resolution Magic Angle Spinning (hr-MAS) is an established technique for analyzing intact biological tissue samples. By spinning at the magic angle, line broadening effects due to dipolar interactions and susceptibility differences within the sample are removed resulting in high resolution quality spectra. hr-MAS probes are configured with a Z-gradient aligned along the magic angle enabling the researcher to access a wide variety of NMR experimental techniques including gradient enhanced solvent suppression and artifact free 2D homo-nuclear and hetero-nuclear experiments. Disposable rotor inserts are available for easy sample preparation and clean up. Here we present some practical recommendations for applying this technique to biological tissue samples.

Sample Analysis:
NMR spectra are typically acquired at 4ºC to slow sample degradation and with a sample spin rate of 4 KHz. 1D 1H spectra with NOESY presaturation and/or 1D 1H CPMG filtered spectra are used for metabolite analysis via AMIX Software. 2D rotor synchronized adiabatic TOCSY and 1H/13C HSQC experiments aid in the identification of the metabolites of the samples.

: 1D CPMG filtered 1H experiment on a 20 mg sample of turtle brain with D2O. A vertical expansion of the downfield region shows low level metoblites present in the sample. Data was acquired at 500 MHz using a 4 mm TXI hr-MAS probe with Zgradient, 64 scans, 4 KHz sample spin rate, 4º C. Sample provided by Dr. Gary Lorigan, Miami University.

: 2D Rotor Synchronized Adiabatic TOCSY with WATERGATE water suppression on a 21 mg sample of mouse kidney Data was acquired at 500 MHz using a 4 mm TXI hr-MAS probe with Zgradient, 2250 Hz sample spin rate. Sample provided by Dr.