MicroProbe Applications

Protein Structure determination

Example spectra from a structure determination of a 8 kD protein. 5 microliters of a 1.4 mM solution were used, corresponding to ca 72 micrograms of protein. Sample courtesy of NESG, Guy Montelione
 

Protein structure determination
HCCH TOCSY = 4 scans, 1k x 64 x 80 complex points, 27 hours
HNCO = 8 scans, 512 x 24 x 48 complex points, 11 hours
15N NOESY-HSQC = 32 scans, 512 x 24 x 80 complex points, 3 days 3 hours