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NMR Inverse Probes
Optimized for 1H applications
The inner coil of these versatile probes is optimized for 1H observation while the outer coil is tuneable over a frequency range that permits decoupling of nuclei between 31P and 15N, depending on probe configuration. You can simply switch between decoupling nuclei using an automated tuning and matching device (ATM).
BBI Broadband Probes
Bruker’s Tuneable Multinuclear Probes are the inverse equivalent of BBO probes and are often called Broadband Inverse Probes. These probes are used extensively for popular experiments like HSQC, HMQC, and HMBC. They are also used for proton 1D experiments with X-Nucleus decoupling and experiments requiring solvent suppression. With the widest tuning range in the industry, both 1D and multidimensional experiments can be performed with a variety of nuclei. The inner coil of Bruker’s BBI Probe is optimized for 1H observation while the outer coil can be tuned from 31P to 15N.
Standard BBI Probes are available as
| Type | Frequency | Diameter | Gradients |
|---|---|---|---|
| BBI | 300 MHz | 5mm | Z-Grad |
| BBI | 400 MHz | 5mm | Z-Grad |
| BBI | 500 MHz | 5mm | Z-Grad |
| BBI | 600 MHz | 5mm | Z-Grad |
| BBI | 700 MHz | 5mm | Z-Grad |
| BBI | 800 MHz | 5mm | Z-Grad |
| BBI | 300-800 MHz | 3mm | Z-Grad |
Triple (TXI) & Quadruple Resonance (QXI) Probes
Bruker’s Triple (TXI) and Quadruple (QXI) Resonance Probes are used extensively for the family of double, triple and quadruple resonance experiments developed to perform NMR structure determination of biological macromolecules. Select a probe with decoupling nuclei to match your sample requirements.
Inverse Triple and Quadruple Resonance Probes give you access to advanced NMR techniques, letting you pulse up to three or four nuclei, respectively, in one experiment. The inner coil of these probes is optimized for 1H observation. For QXI probes, the inner coil is also tuned for decoupling of one specified nucleus. The outer coil of both TXI and QXI probes is optimized for decoupling of two specified nuclei. If you add a deuterium lock switch, you can decouple 2H as well, permitting decoupling of up to three (TXI) or four (QXI) nuclei in a single pulse sequence.
Standard TXI Probes are available as
| Type | Frequency | Diameter | Gradients |
|---|---|---|---|
| TXI | 400 MHz | 1 mm | Z-Gradient |
| TXI | 400 MHz | 1.7mm | Z-Gradient |
| TXI | 400 MHz | 5 mm | Z-Gradient |
| TXI | 500 MHz | 1 mm | Z-Gradient |
| TXI | 500 MHz | 1.7mm | Z-Gradient |
| TXI | 500 MHz | 5 mm | Z-Gradient |
| TXI | 500 MHz | 5 mm | XYZ-Gradient |
| TXI | 600 MHz | 1 mm | Z-Gradient |
| TXI | 600 MHz | 1.7mm | Z-Gradient |
| TXI | 600 MHz | 5 mm | Z-Gradient |
| TXI | 600 MHz | 5 mm | XYZ-Gradient |
| TXI | 700 MHz | 1 mm | Z-Gradient |
| TXI | 700 MHz | 1.7mm | Z-Gradient |
| TXI | 700 MHz | 5 mm | Z-Gradient |
| TXI | 700 MHz | 5 mm | XYZ-Gradient |
| TXI | 800 MHz | 5 mm | Z-Gradient |
| TXI | 900 MHz | 5 mm | Z-Gradient |
| TXI | 850 MHz | 5 mm | Z-Gradient |
| TXI | 950 MHz | 5 mm | Z-Gradient |
Standard QXI Probes are available as
| Type | Frequency | Diameter | Gradients |
|---|---|---|---|
| QXI | 500 MHz | 5 mm | Z-Gradient |
| QXI | 500 MHz | 5 mm | XYZ-Gradient |
| QXI | 600 MHz | 5 mm | Z-Gradient |
| QXI | 600 MHz | 5 mm | XYZ-Gradient |
Tuneable Multinuclear Triple (TBI) Resonance Probes
Bruker’s TBI Probes are used for all the experiments listed for the BBI probe. However, they can also be used for triple resonance experiments. In addition to the well-known 1H, 13C and 15N experiments, these probes can be used for the study of organometallic compounds and metalloproteins using 1H, 13C and another X-Nucleus. These probes extend the application of TXI probes by allowing the selection of one decoupling nucleus from a wide tuning range.
The inner coil of the Bruker’s TBI Probe is optimized for 1H observation and decoupling a fixed nucleus, e.g. 13C. The outer coil can be tuned to one decoupling nucleus from the widest tuning range in the industry, covering 31P to 15N, depending upon configuration. Appropriate amplifiers and RF sources are required to take full advantage of these probes.
Standard TBI Probes are available as
| Type | Frequency | Diameter | Gradients |
|---|---|---|---|
| TBI | 400 MHz | 5 mm | Z-Gradient |
| TBI | 500 MHz | 5 mm | Z-Gradient |
| TBI | 600 MHz | 5 mm | Z-Gradient |
Selective Inverse Probes (non standard)
Selective (SEI) Probes provide the highest sensitivity, best lineshape, and finest resolution in inverse experiments. Bruker SEI probes are favored in LC-NMR, labelled protein screening and other applications which benefit from the lower detection limits of these highly optimized probes. The inner coil of Bruker's SEI Probes is optimized for 1H observation, while the outer coil is optimized for decoupling a specific nucleus, such as 13C or 15N.

