CryoProbe

A World of Applications with Ultimate Sensitivity

The development of CryoProbes delivered the single largest increase in NMR sensitivity in the last few decades. This jump in sensitivity enables scientists to observe sample amounts that were considered too small just a few years ago and to increase sample throughput up to 16- fold.
 

The CryoProbe Concept

Every CryoProbe™ interfaced with a fully automated universal CryoPlatform™ maintains probe electronics at very low temperatures in order to reduce noise contributions. Once in the cold state a CryoProbe can virtually be used like a conventional probe. The sample, while just a few millimeters away from the cold RF coils, is stabilized at a user defined temperature around ambient. The closed-cycled cooling system controls all functions and guarantees excellent stability during short and long term experiments. As a result, the system is easy to use.
 

 Observe CryoProbes (X-nuclei)

 

 Inverse CryoProbes (1H)
 

CryoProbe Mechanics

CryoProbe technology takes advantage of the fact that the radio frequency electronics will generate a higher signal and less noise at lower temperatures. By reducing the temperature of the NMR coil and preamplifier signal to noise ratio, enhancements of a factor of four or more can be achieved. This can easily translate into an order of magnitude higher throughput. The coil assembly and preamplifier are cooled using cold helium gas in a closed-loop cooling system. The closed-loop cooling system, in which helium gas is compressed in one chamber and then allowed to expand in a second chamber, produces the cold helium gas. Vacuum-insulated parts in the cooling system allow the coil assembly to reach very low temperatures. Thermal isolation in the probe is critical to allow samples to be measured at room temperature only a few millimeters from the cold coil assembly.
CryoPlatform
 

Probe Changes

Automatic cool-down, warm-up and timer features of our CryoPanel™ software provides a complete user interface to the closed-loop cooling system, the Cryo- Platform. The one button operation of cool-down and warm-up are completed in 2.5 hours and 1.5 hours, respectively. A timer feature enables the execution of these functions at any predefined time, whether or not the user is present.
 

Sample Handling

Sample handling in a CryoProbe is the same as in a conventional high resolution probe. Sample changers such as the B-ACS™ and NMR Case may be used with CryoProbes for samples in NMR tubes. For flow methods, CryoProbes are compatible with the BEST™ Flow Injection Accessory and may be also used in combination with LC-NMR. CryoFIT™ is available to convert a CryoProbe from tube operation to flow methods.
CryoFit