Novel 1.7 mm Micro-CryoProbes
Greater than tenfold jump in NMR sensitivity for small sample amounts
The new 1.7 mm triple- resonance Micro-CryoProbe has a total volume of 30 microliters and offers an increase in mass sensitivity of more than an order of magnitude compared to a conventional 5 mm probe. This extreme sensitivity jump makes the 1.7 mm Micro-CryoProbe an ideal tool for any NMR analysis with limited sample amounts, e.g. natural products, isolated low abundance proteins, peptides or small molecules, difficult-to-express proteins, etc.
Conventional microprobes with 5 and 30 micro liter volumes have become very popular in areas such as natural products chemistry, protein NMR and drug screening applications. The introduction of a cryogenically cooled Micro-CryoProbe is of significant importance for researchers working with very limited sample quantities. These samples can be natural products isolated in minute quantities from a variety of organisms or protein samples that have been prepared in small scale expression systems or isolated from natural sources.
The combination of the gain achieved by cryogenic cooling both coil and preamplifier and by proprietary high-sensitivity electronics designs leads to this significant increase in sensitivity. For a given sample amount, a 6-fold gain over a conventional 1.7mm probe and about 10-14 fold gain over a conventional 5mm probe are obtained. This can lead to a 200-fold reduction in experiment time, or alternatively it fundamentally enables NMR research with very low sample quantities that previously would simply not have been possible or practical.